Topoisomerase II alpha Antibody / TOP2A

• Catalog Number: orb1825098
• Category: Antibodies
• Description: The topoisomerase II-Alpha isoform is a 170kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis. Topoisomerase II-Alpha is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers. Decreased expression of Topoisomerase II-Alpha is the predominant mechanism of resistance to several chemotherapeutic agents.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: TOP2A/8103R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 1431-1531) from the human protein was used as the immunogen for the Topoisomerase II alpha antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Topoisomerase II alpha antibody is available for research use only.
• UniProt ID: P11388
• Storage: Aliquot the Topoisomerase II alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lymph node tissue with Topoisomerase II alpha antibody (clone TOP2A/8103R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Topoisomerase II alpha antibody (clone TOP2A/8103R) as confirmation of integrity and purity.