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Catalog Number | orb1825092 |
---|---|
Category | Antibodies |
Description | The topoisomerase II-Alpha isoform is a 170kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis. Topoisomerase II-Alpha is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers. Decreased expression of Topoisomerase II-Alpha is the predominant mechanism of resistance to several chemotherapeutic agents. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | TOP2A/7169R |
Tested applications | IHC-P |
Reactivity | Canine, Human |
Isotype | Rabbit IgG, kappa |
Immunogen | A recombinant partial protein sequence (within amino acids 1431-1531) from the human protein was used as the immunogen for the Topoisomerase II alpha antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This Topoisomerase II alpha antibody is available for research use only. |
UniProt ID | P11388 |
Storage | Aliquot the Topoisomerase II alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE dog lymph node tissue with Topoisomerase II alpha antibody (clone TOP2A/7169R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with Topoisomerase II alpha antibody (clone TOP2A/7169R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Canine, Human | |
Rabbit | |
Recombinant | |
Unconjugated |
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