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Catalog Number | orb1151736 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to Topoisomerase II alpha |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rTOP2A/6629 |
Tested applications | IHC-P |
Reactivity | Human, Mouse |
Isotype | Mouse IgG1, kappa |
Immunogen | A portion of amino acids 1352-1493 was used as the immunogen for the recombinant Topoisomerase II alpha antibody. |
Dilution range | Immunohistochemistry (FFPE): 0.5-1ug/ml |
Purity | Protein A/G affinity |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant Topoisomerase II alpha antibody is available for research use only. |
UniProt ID | P11388 |
Storage | Aliquot the recombinant Topoisomerase II alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant Topoisomerase II alpha antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human lymph node with recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629) as confirmation of integrity and purity.
IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC-P, WB | |
Human, Mouse | |
Mouse | |
Recombinant | |
Unconjugated |
IF, IH, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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