Topo II alpha antibody

• Catalog Number: orb766503
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Topo II alpha
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Monkey
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human TOP2A. AA range:1-50
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 174
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-TOP2A antibody, anti-TOP2 antibody, anti-DNA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Topo IIα Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Western Blot analysis of 293 cells using Topo IIα Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Immunofluorescence analysis of A549 cells, using TOP2A Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using TOP2A Antibody. The picture on the right is blocked with the synthesized peptide.