Topo II Alpha Antibody

• Catalog Number: orb1530753
• Category: Antibodies
• Description: Topo II Alpha Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 1481 and C-terminus
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:30,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Topo II Alpha
• UniProt ID: P11388
• NCBI: NP_001058.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: DNA topoisomerase II, alpha isozyme; TP2A; DNA gyr
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human TOPO-II-Alpha by western blot.Samples: Whole cell lysate from Jurkat, HeLa, and HEK293T cells. Antibody: Affinity purified rabbit anti-TOPO-II-Alpha antibody orb1530753 at 0.04 µg/ml.

Detection of human Topo II Alpha by immunohistochemistry. Sample: FFPE section of human laryngeal squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Topo II alpha used at a dilution of 1:1, 000 (1µg/ml).

Detection of human TOPO-II-Alpha by western blot of immunoprecipitates.Samples: Whole cell lysate from Jurkat cells. Antibodies: Affinity purified rabbit anti-TOPO-II-Alpha antibody.