TOP1MT Antibody / Topoisomerase I mitochondrial

• Catalog Number: orb749467
• Category: Antibodies
• Description: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TOP1MT/488
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the TOP1MT antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This TOP1MT antibody is available for research use only.
• UniProt ID: Q969P6
• Storage: Store the TOP1MT antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the TOP1MT antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human MCF7 cells with TOP1MT antibody (clone TOP1MT/488, green) and Reddot nuclear stain (red).

IHC: Formalin-fixed, paraffin-embedded human melanoma stained with TOP1MT antibody (clone TOP1MT/488).

IHC: Formalin-fixed, paraffin-embedded human gallbladder stained with TOP1MT antibody (clone TOP1MT/488).

Flow cytometry testing of PFA-fixed human MCF7 cells with TOP1MT antibody (clone TOP1MT/488); Red=isotype control, Blue=TOP1MT antibody.

Western blot testing of human Jurkat cell lysate with TOP1MT antibody (clone TOP1MT/488). Predicted molecular weight ~66 kDa.

SDS-PAGE analysis of purified, BSA-free TOP1MT antibody (clone TOP1MT/488) as confirmation of integrity and purity.

IHC: Formalin-fixed, paraffin-embedded human pancreas stained with TOP1MT antibody (clone TOP1MT/488).