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Catalog Number | orb1272770 |
---|---|
Category | Antibodies |
Description | TNFSF9 Antibody (Biotin) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant h4-1BBL (human 4-1BBLigand). Human 4-1BBL specific antibody was purified by affinity chromatography and then biotinylated. |
Concentration | batch dependent |
Dilution range | ELISA:Sandwich:To detect h4-1BBL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human 4-1BBL as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant h4-1BBL. Western Blot:To detect h4-1BBL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant h4-1BBL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Biotin |
Target | TNFSF9 |
UniProt ID | P41273 |
NCBI | P41273 |
Storage | 4-1BBL antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | CD137L, 4-1BB-LTumor necrosis factor ligand superf Read more... |
Note | For research use only |
Application notes | ELISA:Sandwich:To detect h4-1BBL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human 4-1BBL as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant h4-1BBL. Western Blot:To detect h4-1BBL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant h4-1BBL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
ELISA | |
Human | |
Rabbit | |
Polyclonal | |
Biotin |
ELISA, ICC, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Biotin |
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