TNFSF15 Antibody / VEGI / TL1A

• Catalog Number: orb376640
• Category: Antibodies
• Description: Vascular endothelial growth inhibitor (VEGI), also known as TNF-like ligand 1A (TL1A) and TNF superfamily member 15 (TNFSF15), is protein that in humans is encoded by the TNFSF15 gene. TL1A/VEGI is an anti-angiogenic protein. It belongs to tumor necrosis factor (ligand) superfamily, where it is member 15. It is the sole known ligand for death receptor 3, and it can also be recognized by decoy receptor 3.TL1A is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF-alpha and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 (death receptor 3) and TNFRSF6B (decoy receptor 3). It can activate both the NF-κB and MAPK signalling pathways, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. [Wiki]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TLRM1-1
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A full length human recombinant protein was used as the immunogen for this TL1A antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This TL1A antibody is available for research use only.
• Entrez: 9966
• UniProt ID: O95150
• Storage: Store the TL1A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TL1A antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human parathyroid tumor with TL1A antibody (clone TLRM1-1). Staining of FFPE tissue requires boiling sections in 10 mM Tris with 1 mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human colon carcinoma with TL1A antibody (clone TLRM1-1). Staining of FFPE tissue requires boiling sections in 10 mM Tris with 1 mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.