TNFSF13B Antibody

• Catalog Number: orb1272250
• Category: Antibodies
• Description: TNFSF13B Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2B
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The monoclonal antibody was produced in BALB/c mice using recombinant human BAFF as the immunizing antigen.
• Concentration: batch dependent
• Dilution range: ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 5.0-6.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human BAFF when used with our biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 μg/mL. Western Blot:To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: TNFSF13B
• UniProt ID: Q9Y275
• NCBI: Q9Y275
• Storage: BAFF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
• Alternative names: B cell Activating Factor , BAFF, BLYS, TALL1, TNFS
• Note: For research use only
• Application notes: ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 5.0-6.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human BAFF when used with our biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 μg/mL. Western Blot:To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To a sandwich ELISA (assuming 100ul/well), a concentration of 5.0-6.0 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human BAFF when used with antigen affinity purified anti-human BAFF (orb1272594) as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml.

To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.

To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.