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Catalog Number | orb1228200 |
---|---|
Category | Proteins |
Description | TNFRSF9 Recombinant Protein |
Tested applications | WB |
Tag | C-Fc Tag |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE. |
MW | 43.3 kDa |
Target | TNFRSF9 |
UniProt ID | Q07011 |
Protein Sequence | Gln 25 - Gln 186 |
Source | HEK293 cells |
Biological Activity | Measured by its binding ability in a functional ELISA. When Recombinant Human 4-1BB Protein, With C-Fc Tag is Immobilized at 10 ng/mL (100 μl/well), Recombinant Human 4-1BB Ligand produces 50% optimal binding response with a concentration range of 0. 75 – 3. 0 ng/mL. |
NCBI | NP_001552.2 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 50 mM tris, 100 mM glycine, pH 7.5. |
Alternative names | TNFRSF9, 4-1BB, CD137, CDw137, ILA Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for WB. For research use only. |
Expiration Date | 6 months from date of receipt. |
The purity of rh CD137 Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
ELISA, WB | |
> 95% as determined by SDS-PAGE. | |
44.2 kDa | |
HEK293 cells |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 43.4 kDa after removal of the signal peptide. The apparent molecular mass of c4-1BB-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
19.2 kDa | |
HEK293 cells |