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Catalog Number | orb1239336 |
---|---|
Category | Tools |
Description | TNFRSF25 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, WB |
Isotype | IgG |
Immunogen | DR3 antibody was raised against a peptide corresponding to amino acids in extracellular domain of human DR3 precusor.The immunogen is located within amino acids 50 - 100 of DR3. |
Concentration | 1 mg/ml |
Dilution range | DR3 antibody can be used for detection of DR3 expression by Western blot at 1 μg/mL. 59 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 59 kDa |
Target | TNFRSF25 |
UniProt ID | Q93038 |
NCBI | AAQ88676 |
Storage | DR3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | DR3 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | DR3 Antibody: DR3, TR3, DDR3, LARD, APO-3, TRAMP, Read more... |
Note | For research use only |
Application notes | DR3 antibody can be used for detection of DR3 expression by Western blot at 1 μg/mL. 59 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:1000 dilution.
Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 µg/mL.
Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 µg/mL.
Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 µg/mL. Green: DR3 Antibody (orb1239336) Blue: DAPI staining.
Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 µg/mL.
ICC, IF, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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