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Catalog Number | orb1227855 |
---|---|
Category | Proteins |
Description | TNFRII Recombinant Protein |
Tested applications | WB |
Tag | His Tag |
Dilution range | This recombinant protein can be used for WB. For research use only. |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE. |
MW | 26 kDa |
Target | TNFRSF1B |
UniProt ID | P20333 |
Protein Sequence | Leu 23 - Asp 257 |
Source | HEK293 cells |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized human TNFa at 5μg/mL (100μl/well) can bind human TNFR2. The EC50 of human TNFR2 is 647 ng/mL. |
NCBI | AAA36755 |
Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | TNFRSF1B, CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for WB. For research use only. |
Expiration Date | 6 months from date of receipt. |
Human TNFR2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 26.2 kDa after removal of the signal peptide. The apparent molecular mass of mTNFRSF1B-His is approximately 25-55 kDa due to glycosylation. | |
Mammalian |
≥90% as determined by SDS-PAGE | |
This protein contains the mouse Tnfrsf1b(Val23-Gly258) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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