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Catalog Number | orb1227857 |
---|---|
Category | Proteins |
Description | TNFR1 Recombinant Protein |
Tested applications | WB |
Tag | C-Fc Tag |
Form/Appearance | Lyophilized |
Purity | > 98% as determined by SDS-PAGE. |
MW | 47.9 kDa |
Target | TNFRSF1A |
UniProt ID | P19438 |
Protein Sequence | Ile 22 - Thr 211 |
Source | HEK293 cells |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human TNFR1/CD120a Protein,C-Fc Tag at 5 μg/mL (100 μl/well) can bind Biotinylated Human TNF-alpha Protein . The EC50 is typically 2. 8-11. 4 ng/mL. |
NCBI | NP_001056 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 50 mM tris, 100 mM glycine, pH 7.5 |
Alternative names | TNFR1, CD120a, TNFRSF1A, FPF, TBP1, TNF-R, TNF-R-I Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for WB. For research use only. |
Expiration Date | 6 months from date of receipt. |
The purity of rh TNFR1 Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
Greater than 85% as determined by SDS-PAGE. | |
22.7 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
41.2 kDa | |
E.coli |
Greater than 85% as determined by SDS-PAGE. | |
41.6 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |