TNF alpha Recombinant Protein (HEK293 Expressed)

• Catalog Number: orb1227854
• Category: Proteins
• Description: TNF alpha Recombinant Protein (HEK293 Expressed)
• Tested applications: WB
• Tag: Tag free
• Form/Appearance: Lyophilized
• Purity: > 95% as determined by SDS-PAGE.
• MW: 17.4 kDa
• Target: TNF
• UniProt ID: P01375
• Protein Sequence: Val 77 - Leu 233
• Source: HEK293 cells
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha at 5 ug/mL (100 ul/well) can bind Humira with a linear range of 0.04-0.6 ng/mL. The bio-activity was determined by its cytotoxcity assy using L929 Cells with 1ug/ml actinomycin D.
• NCBI: P01375
• Storage: Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Alternative names: DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin,
• Note: For research use only
• Application notes: This recombinant protein can be used for WB. For research use only.
• Expiration Date: 6 months from date of receipt.

Human TNF-alpha, Tag Free, low endotoxin (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.