TNF-alpha Recombinant Protein

• Catalog Number: orb1227719
• Category: Proteins
• Description: TNF-alpha Recombinant Protein
• Tested applications: ELISA, WB
• Tag: His Tag
• Form/Appearance: Lyophilized
• Purity: > 95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method.
• MW: 18.2 kDa
• Target: TNF-alpha
• Protein Sequence: Val 77 - Leu 233
• Source: HEK293 cells
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha, His Tag at 0.2 ug/mL (100 uL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.4-6.25 ng/mL.
• NCBI: NP_000585.2
• Storage: Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Alternative names: DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFal
• Note: For research use only
• Application notes: This recombinant protein can be used for E, WB. For research use only.
• Expiration Date: 6 months from date of receipt.

Immobilized Human TNF-alpha, His Tag at 0.2 ug/ml (100 uL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.4-6.25 ng/mL.