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Catalog Number | orb7100 |
---|---|
Category | Antibodies |
Description | TNF alpha Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Rat |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human TNF-alpha (181-235/235aa) |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, ELISA=1:5000-10000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 17/26 kDa |
Target | TNF |
UniProt ID | P01375 |
RRID | AB_10929448 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | Tumor necrosis factor-α; TNFalpha; APC1; Cachectin Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
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Oh, Ha Na et al. Effects of Quercetin in a Mouse Model of Experimental Dry Eye Cornea, 34, 1130-1136 (2015)
Applications
Reactivity
Correia, Marcelo Henrique et al. Immune response and Raman scattering assessment in rats skin after contact with Fusarium oxysporum metabolites Spectrochim Acta A Mol Biomol Spectrosc, 234, 118246 (2020)
Applications
Reactivity
Shi, Xiaowei et al. Panax notoginseng saponins administration modulates pro- /anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats Metab Brain Dis, 32, 221-233 (2017)
Applications
Reactivity
Sample: Diseased lung (mouse) at 30 ug, Primary: Anti-TNF alpha (orb7100) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000 dilution, Predicted band size: 17/28 kD, Observed band size: 17/28 kD.
Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-TNF alpha (orb7100) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36/17/26 kD, Observed band size: 36 kD.
Sample: Liver (mouse) Lysate at 40 ug, Primary: Anti-TNF alpha (orb7100) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17/26kD, Observed band size: 25 kD.
FC, WB | |
Mouse | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, WB | |
Bovine, Canine, Equine, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, WB | |
Canine, Equine, Porcine | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |