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TNF alpha Rabbit Polyclonal Antibody

Catalog Number: orb7100

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb7100
CategoryAntibodies
DescriptionTNF alpha Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, WB
Predicted ReactivityRat
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human TNF-alpha (181-235/235aa)
Concentration1mg/ml
Dilution rangeWB=1:500-2000, ELISA=1:5000-10000
Form/AppearanceLiquid
ConjugationUnconjugated
MW17/26 kDa
TargetTNF
UniProt IDP01375
RRIDAB_10929448
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesTumor necrosis factor-α; TNFalpha; APC1; Cachectin
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NoteFor research use only
Expiration Date12 months from date of receipt.

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Applications

IHC

Reactivity

Mouse

Correia, Marcelo Henrique et al. Immune response and Raman scattering assessment in rats skin after contact with Fusarium oxysporum metabolites Spectrochim Acta A Mol Biomol Spectrosc, 234, 118246 (2020)

Applications

IHC

Reactivity

Rat
TNF alpha Rabbit Polyclonal Antibody

Sample: Diseased lung (mouse) at 30 ug, Primary: Anti-TNF alpha (orb7100) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000 dilution, Predicted band size: 17/28 kD, Observed band size: 17/28 kD.

TNF alpha Rabbit Polyclonal Antibody

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-TNF alpha (orb7100) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36/17/26 kD, Observed band size: 36 kD.

TNF alpha Rabbit Polyclonal Antibody

Sample: Liver (mouse) Lysate at 40 ug, Primary: Anti-TNF alpha (orb7100) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17/26kD, Observed band size: 25 kD.

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