TNF alpha Antibody

• Catalog Number: orb749824
• Category: Antibodies
• Description: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TNF706
• Tested applications: IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgM, kappa
• Immunogen: An N-terminal recombinant protein fragment was used as the immunogen for the TNFa antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Purity: PEG precipitation
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This TNFa antibody is available for research use only.
• UniProt ID: P01375
• Storage: Store the TNFa antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the TNFa antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded rat pancreas stained with TNFa antibody (clone TNF706).

Immunofluorescent staining of human HepG2 cells with TNFa antibody (green, clone TNF706) and Reddot nuclear stain (red).

IHC: Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNFa antibody (clone TNF706).