TNF alpha Antibody

• Catalog Number: orb606510
• Category: Antibodies
• Description: This mAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: TNF/1500R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the recombinant TNFa antibody.
• Dilution range: Immunohistochemistry (FFPE): 4-8ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant TNFa antibody is available for research use only.
• UniProt ID: P01375
• Storage: Store the recombinant TNFa antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant TNFa antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pancreas with recombinant TNFa antibody (clone TNF/1500R). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

IHC testing of FFPE human histiocytoma with recombinant TNFa antibody (clone TNF/1500R). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant TNFa antibody (clone TNF/1500R) as confirmation of integrity and purity.