TMEPAI Antibody / PMEPA1

• Catalog Number: orb534362
• Category: Antibodies
• Description: PMEPA1 (prostate transmembrane protein, androgen induced 1), or TMEPAI (Transmembrane prostate androgen induced protein) is a 287 amino acid single-pass membrane protein that contains WW-binding motifs and localizes to the cell membrane. Expressed at high levels in prostate, kidney and ovary, PMEPA1 interacts with NEDD4 and may play a role in regulating AR (androgen receptor) levels, specifically in prostate cells. Down regulation of PMEPA1 is observed in prostate tumors, suggesting that PMEPA1 may exhibit activity as a tumor suppressor. Overexpression of this protein may play a role in multiple types of cancer.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PTAI1-1
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: Full-length recombinant human protein was used as the immunogen for the PMEPA1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This PMEPA1 antibody is available for research use only.
• UniProt ID: Q969W9
• Storage: Store the PMEPA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the PMEPA1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human prostate carcinoma with PMEPA1 antibody (clone PTAI1-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.