TLR9 Antibody

• Catalog Number: orb248106
• Category: Antibodies
• Description: The mammalian immune system is composed of two components: innate immunity and adaptive immunity. Toll-like receptors (TLRs) are an essential part of the innate immune system. Through recognition of pathogen-associated molecular patterns (PAMPs), TLRs mount an immune defense against pathogens. There are 10 mammalian TLRs (TLR1-10). Unmethylated CpG sequences in bacterial genomes and viral DNA molecules activates TLR9, leading to the inflammatory response.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TR9541
• Tested applications: FACS, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant protein fragment from the N-terminal portion of human TLR9 was used as the immunogen for this antibody.
• Dilution range: Western blot: 2-4ug/ml,Flow Cytometry: 1ug/million cells,Immunohistochemistry (FFPE): 5ug/ml
• Purity: Protein G Affinity Chromatography
• Conjugation: Unconjugated
• Formula: 0.5 mg/ml antibody in PBS with 0.1 mg/ml BSA (US source) and 0.05% sodium azide
• Hazard Information: This TLR9 antibody is available for research use only.
• Entrez: 54106
• Storage: Aliquot the TLR9 antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Note: For research use only
• Application notes: Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human samples with TLR9 antibody at 2 ug/ml.

Western blot testing of mouse samples with TLR9 antibody at 2 ug/ml.

IHC testing of FFPE human stomach tissue with TLR9 antibody at 5 ug/ml. HIER: steam tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

FACS testing of human PBMC (lymphocytes) with TLR9 antibody at 0.5ug/10^6 cells. Green: isotype control; Red: TLR9 antibody.