TLE1 Antibody

• Catalog Number: orb606851
• Category: Antibodies
• Description: Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein 1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: TLE1/2946R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amino acids 175-338 from the human protein was used as the immunogen for the recombinant TLE1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant TLE1 antibody is available for research use only.
• UniProt ID: Q04724
• Storage: Store the recombinant TLE1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant TLE1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free recombinant TLE1 antibody (clone TLE1/2946R) as confirmation of integrity and purity.

IHC testing of FFPE human endometrial carcinoma with recombinant TLE1 antibody (clone TLE1/2946R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.