TLE1 Antibody

• Catalog Number: orb1822736
• Category: Antibodies
• Description: Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: TLE1/8007R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant human protein fragment (aa 175-338) was used as the immunogen for the Transducin-like enhancer protein 1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This TLE1 antibody is available for research use only.
• UniProt ID: Q04724
• Storage: Aliquot the Transducin-like enhancer protein 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human prostate carcinoma tissue with Transducin-like enhancer protein 1 antibody (clone TLE1/8007R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human bladder tissue with Transducin-like enhancer protein 1 antibody (clone TLE1/8007R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.