TIP47 antibody

• Catalog Number: orb1474150
• Category: Antibodies
• Description: Mouse monoclonal antibody to TIP47
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide of human TIP47. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50)
• Form/Appearance: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PLIN3
• Entrez: 10226
• UniProt ID: O60664
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: M6PRBP1; TIP47; Perilipin-3; 47 kDa mannose 6-phos
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TIP47 expression in Hela (A), K562 (B), Daudi (C) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)

Immunofluorescent analysis of TIP47 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).