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Catalog Number | orb1474149 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to TIP47 |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | FC, WB |
Reactivity | Human |
Immunogen | KLH-conjugated synthetic peptide of human TIP47. The exact sequence is proprietary. |
Dilution range | WB (1/500 - 1/1000), FC (1/10 - 1/50) |
Form/Appearance | Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | PLIN3 |
Entrez | 10226 |
UniProt ID | O60664 |
Source | Mouse |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | M6PRBP1; TIP47; Perilipin-3; 47 kDa mannose 6-phos Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of TIP47 expression in Hela (A), K562 (B), Daudi (C) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)
Flow cytometric analysis of HUVEC cells using Anti-TIP47 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
ELISA, IHC-P, WB | |
Porcine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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