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Catalog Number | orb344479 |
---|---|
Category | Antibodies |
Description | TIMP4 antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 9:4-7 |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Isotype | IgG2b |
Immunogen | Anti-TIMP4 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with full length human TIMP4 protein followed by hybridoma development. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 5-10µg/mL, WB: 1:2,000 to 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-TIMP4 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. TIMP4 antibody is specific for human TIMP4 protein. This antibody reacts with human TIMP4 in tissue sections of formalin-fixed, paraffin-embedded In ELISAs and Native-PAGE, this antibody does not cross-react with recombinant human TIMP-1, -2 or -3. A BLAST analysis was used to suggest cross-reactivity with TIMP4 from human and mouse sources based on the immunizing sequence. Cross-reactivity with TIMP-4 from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q99727 |
NCBI | NM_003256.3 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | mouse anti-TIMP4 antibody, Tissue inhibitor of met Read more... |
Note | For research use only |
Application notes | Anti-TIMP4 antibody has been tested for use in ELISA, Native - Western, and IHC (P). SDS-PAGE - Western Blot not useful for endogenous detection. Specific conditions for reactivity should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry of Mouse Anti-TIMP4 Antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: a) isotype specific IgG2bk monoclonal; b) monoclonal TIMP-4 antibody (clone 9:4-7); and c) polyclonal TIMP-4, each at 10 µg/mL. Secondary antibody: Peroxidase secondary antibody at 1:10000 for 45 min at RT. Localization: TIMP-4 is secreted. Staining: TIMP-4 as precipitated brown signal.
Immunohistochemistry of Mouse anti-TIMP4 antibody. Tissue: human small intestine. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TIMP4 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10000 for 45 min at RT. Staining: TIMP4 as precipitated red signal with hematoxylin purple nuclear counterstain.
Immunohistochemistry of Mouse anti-TMIP4 antibody. Tissue: human kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TIMP4 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10000 for 45 min at RT. Staining: TIMP4 as precipitated red signal with hematoxylin purple nuclear counterstain.
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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