TIMP-1 rabbit pAb

• Catalog Number: orb766484
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TIMP-1
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rabbit, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human TIMP1. AA range:61-110
• Concentration: 1 mg/ml
• Dilution range: IF: 1:50-200 Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 24
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-TIMP1 antibody, anti-CLGI antibody, anti-TIMP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Western blot analysis of lysates from 1) SH-SY5Y, 2) 293, 3) rat brain cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) Actin β Monoclonal Antibody (5B7) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour.

Immunofluorescence analysis of human-uterus tissue. 1, TIMP-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of human-uterus tissue. 1, TIMP-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.