TIM3 antibody

• Catalog Number: orb2276373
• Category: Antibodies
• Description: TIM3 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-366R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: Q8TDQ0
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: CD366; HAVR2; Hepatitis A virus cellular receptor
• Note: For research use only
• Application notes: Positive Control: Tonsil: A moderate to strong TIM-3 immunostaining should be seen in a significant fraction of T-cells, while only few TIM-3 positive cells are seen in the germinal centre and the mantle zone. Negative Control: Tonsil: Epithelial cells should be negative. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Alveolar macrophages show strong TIM 3 immunostaining.

Colon descendens, mucosa: In the colon mucosa, variable levels of TIM-3 immunostaining are seen in T-cells and in macrophages. Epithelial staining does not occur.

Uterus cervix TIM3 negative squamous cell carcinoma showing a large number of TIM3 positive macrophages and also lymphocytes.