TIM-3 Detection Set (Risk Free)

• Catalog Number: orb1227436
• Category: Assays and Kits
• Description: TIM-3 Detection Set (Risk Free)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, IgG2b
• Immunogen: TIM-3 antibody was raised against the extracellular domain of human TIM-3
• Concentration: Antibody 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PBS containing 0.02% sodium azide.
• Note: For research use only
• Expiration Date: Please enquire.

Titration curve analysis of TIM-3 mAbs to detect recombinant TIM-3 in ELISA with orb1240153, orb1238174, orb1240159, orb1240157, orb1240154, orb1240156, and orb1240178 antibodies at decreasing concentrations.

Western blot analysis of TIM-3 in over expressing HEK293 cells using orb1240153, orb1240157, and orb1240154 antibodies at (A) 0.25 μg/ml, (B) 0.5 μg/ml, and (C) 1 μg/ml.

Immunocytochemistry of TIM-3 in over expressing HEK293 cells using (A) orb1240153, (B) orb1238174, (C) orb1240159, (D) orb1240157, (E) orb1240154, (F) orb1240156, (G) orb1240178, and (H) control mouse IgG antibody at 1 μg/ml.

Immunofluorescence of TIM-3 in over expressing HEK293 cells using (A) orb1240153, (B) orb1240159, (C) orb1240157, (D) orb1240154, (E) orb1240156, (F) orb1240178, and (G) control mouse IgG antibody at 10 μg/ml.

Immunofluorescence of TIM-3 in human colon carcinoma tissue using (A) orb1240153, (B) orb1240159, (C) orb1240157, (D) orb1240154, (E) orb1240156, (F) orb1240178, and (G) control mouse IgG antibody at 20 μg/ml.

Immunohistochemistry of TIM-3 in human colon carcinoma tissue using (A) orb1240153, (B) orb1240159, (C) orb1240157, (D) orb1240154, (E) orb1240156, (F) orb1240178, and (G) control mouse IgG antibody at 5 μg/ml.

Flow cytometry analysis of TIM-3 in over expressing HEK293 cells using (A) orb1240153, (B) orb1238174, (C) orb1240159, (D) orb1240157, (E) orb1240154, (F) orb1240156, (G) orb1240178, and (H) mouse IgG at 0.1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIM-3 over expressing HEK293 cells.