TIGIT Antibody [1B4], Rabbit IgG

• Catalog Number: orb1671717
• Category: Antibodies
• Description: TIGIT Antibody [1B4], Rabbit IgG
• Clonality: Recombinant
• Clone Number: 1B4
• Tested applications: Blocking, ELISA, FC
• Reactivity: Mouse
• Isotype: IgG lambda
• Immunogen: This antibody was raised by immunising TIGIT-/- mice with recombinant murine TIGIT tetramers.
• Concentration: batch dependent
• Conjugation: Unconjugated
• UniProt ID: P86176
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: VSTM3, T cell Ig and ITIM domain, T cell immunorec
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-TIGIT antibody 1B4. RAW-264.7 cells (A)- mouse spleen (B) and mouse thymus (C) lysates (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric r version of 1B4 (orb1671717) at 0.3 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Binding curve of anti-TIGIT antibody 1B4 (orb1671717) to recombinant mouse TIGIT Fc-Fusion Protein. ELISA Plate coated with recombinant mouse recombinant mouse TIGIT Fc-Fusion Protein (Pr00163-1.9; Antibody) at a concentration of 2 ug/ml. A 4-fold serial dilution from 5-000 ng/ml was performed using orb1671717. For detection- a 1:4000 dilution of HRP-lnti-ribody was used.