Tie-2 (phospho Tyr992) rabbit pAb

• Catalog Number: orb767261
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Tie-2 (phospho-Tyr992)
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Synthesized phospho-peptide around the phosphorylation site of human Tie-2 (phospho Tyr992)
• Concentration: 1 mg/ml
• Dilution range: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
• Conjugation: Unconjugated
• MW: 125
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-TEK antibody, anti-TIE2 antibody, anti-VMCM a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of VEC cells using Phospho-Tie-2 (Y992) Polyclonal Antibody.

Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Western Blot analysis of mouse lung, mouse liver , using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000.