Tie-2 (phospho Tyr1108) rabbit pAb

• Catalog Number: orb770239
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Tie-2 (phospho-Tyr1108)
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human TIE2 around the phosphorylation site of Tyr1108. AA range:1074-1123
• Concentration: 1 mg/ml
• Dilution range: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
• Conjugation: Unconjugated
• MW: 150
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-TEK antibody, anti-TIE2 antibody, anti-VMCM a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TIE2 (Phospho-Tyr1108) Antibody.

Immunofluorescence analysis of HepG2 cells, using TIE2 (Phospho-Tyr1108) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from NIH/3T3 cells, using TIE2 (Phospho-Tyr1108) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).