Thyroglobulin antibody

• Catalog Number: orb344416
• Category: Antibodies
• Description: Thyroglobulin antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2H11
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with human thyroglobulin protein.
• Concentration: 1.6 mg/ml
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:50 - 1:200, WB: 1:50 - 1:200
• Form/Appearance: Liquid (sterile filtered)
• Purity: This protein A purified mouse monoclonal antibody reacts specifically with thyroglobulin in human tissues. The antibody recognizes a 330-kDa band corresponding to thyroglobulin. Cross reactivity with thyroglobulin from mouse, rat and dog will occur. Cross reactivity with thyroglobulin from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P01266
• NCBI: P01266.5
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-Thyroglobulin Antibody, AITD 3 antibody
• Note: For research use only
• Application notes: Anti-Thyroglobulin antibody has been tested by western blot and is suitable for the detection of thyroglobulin in ELISA, immunohistochemistry, and immunoprecipitation. For immunohistochemistry, both frozen sections and formalin fixed, paraffin-embedded tissue sections can be used without epitope retrieval or enzyme digestion. This antibody is specific for the 330 kDa thyroglobulin protein. Thyroglobulin shows a cytoplasmic localization. Thyroid tissue can be used as a positive control.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 µg, right lane 3 ng) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2 h followed by detection using a 1:5000 dilution of IRDye™800 conjugated Goat-a-Mouse IgG [H&L].