Thymidylate Synthase Antibody

• Catalog Number: orb639548
• Category: Antibodies
• Description: Thymidylate synthase converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. Thymidylate synthase is also a critical target for the fluoropyrimidines, an important group of anti-neoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates thymidylate synthase by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of thymidylate synthase protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TYSY9-2
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: A recombinant human protein from within amino acids 60-174 was used as the immunogen for this Thymidylate synthase antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G purified monoclonal antibody
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Thymidylate Synthase antibody is available for research use only.
• Entrez: 7298
• Storage: Store the Thymidylate synthase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil and Thymidylate Synthase antibody (clone TYSY9-2). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.

Immunofluorescent staining of PFA-fixed human Ramos cells with Thymidylate Synthase antibody cocktail (green, clone TYSY9-2) and Phalloidin (red).

Western blot testing of human HeLa cell lysate with Thymidylate Synthase antibody (clone TYSY9-2). Predicted molecular weight ~36 kDa.