THB(Tetrahydrobiopterin) ELISA Kit

• Catalog Number: orb1088240
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Tetrahydrobiopterin (THB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tetrahydrobiopterin (THB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tetrahydrobiopterin (THB) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: All
• Range: 1.57-100 ng/mL
• Sensitivity: 0.51 ng/mL
• Protein Length: 2h
• Alternative names: Tetrahydrobiopterin, BH4, Kuvan, Sapropterin
• Note: For research use only
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.