TGFBR2 Antibody (N-term)

• Catalog Number: orb1937905
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB31405
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:1000, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 64568 Da
• Target: This TGFBR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-40 amino acids from the N-terminal region of human TGFBR2.
• UniProt ID: P37173
• NCBI: NP_001020018.1, NP_003233.4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: TGF-beta receptor type-2, TGFR-2, TGF-beta type II
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution. Lane 1: human testis lysate. Lane 2: mouse lung lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution. Lane 1: NCCIT whole cell lysates. Lane 2: mouse lung lysates. Lane 3: SW620 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution + mouse lung lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution + mouse lung lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution + mouse lung lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of TGFBR2 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

TGFBR2 Antibody (N-term) western blot analysis in mouse lung tissue lysates (35 ug/lane). This demonstrates the TGFBR2 antibody detected the TGFBR2 protein (arrow).