TGF beta Receptor I Mouse Monoclonal Antibody

• Catalog Number: orb1040886
• Category: Antibodies
• Description: TGF beta Receptor I Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5A8
• Tested applications: IF, IHC-Fr, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, Kappa
• Immunogen: KLH conjugated synthetic peptide derived from human TGF beta Receptor I
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: TGFBR1
• UniProt ID: P36897
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: TGF-beta receptor type-1; TGFBR1; TGF Beta R1; AAT
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TGF beta Receptor I) Monoclonal Antibody, Unconjugated (orb1040886) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TGF beta Receptor I) Monoclonal Antibody, Unconjugated (orb1040886) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TGF beta Receptor I) Monoclonal Antibody, Unconjugated (orb1040886) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.