TGF beta 1 Rabbit Polyclonal Antibody

• Catalog Number: orb7087
• Category: Antibodies
• Description: TGF beta 1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Guinea pig, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TGF-Beta 1 (351-390/390aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 12.8/44 kDa
• Target: TGFB1
• UniProt ID: P01137
• RRID: AB_10939710
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CED; DPD1; TGF beta 1; TGF beta; TGF beta 1 protei
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Jin, Jin et al. Corrigendum: Lipoxin A4 Inhibits NLRP3 Inflammasome Activation in Rats With Non-compressive Disc Herniation Through the JNK1/Beclin-1/PI3KC3 Pathway Front Neurosci, 14, 608184 (2020)

PubMed: 33312119

Shang, Peijin et al. Acetyl-11-Keto-β-Boswellic Acid Attenuates Prooxidant and Profibrotic Mechanisms Involving Transforming Growth Factor-β1, and Improves Vascular Remodeling in Spontaneously Hypertensive Rats Sci Rep, 6, 39809 (2016)

PubMed: 28009003

Junga, Anna et al. The Morphopathogenetic Aspects of Intraabdominal Adhesions in Children under One Year of Age Medicina (Kaunas), 55, E556 (2019)

PubMed: 31480453

Niwa, Takahiko et al. The dynamics of TGF-β in dental pulp, odontoblasts and dentin Sci Rep, 8, 4450 (2018)

PubMed: 29535349

Buile, Dace et al. Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients Pediatr Rep, 14, 419-433 (2022)

PubMed: 36278554

Blank control: MG63. Primary Antibody (green line): Rabbit Anti-TGF beta 1 antibody (orb7087), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TGF beta 1) Polyclonal Antibody, Unconjugated (orb7087) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Sample: Embryo (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-TGF beta 1 (orb7087) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 12.8/44 kD, Observed band size: 59 kD.

Sample: Lane 1: Stomach (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Placenta (Mouse) Lysate at 40 ug, Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 5: Liver (Rat) Lysate at 40 ug, Primary: Anti-TGF beta 1 (orb7087) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 12.8/44 kDa, Observed band size: 50 kD.

Sample: Ovary (Mouse) Lysate at 40 ug, Primary: Anti-TGF beta 1 (orb7087) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 12.8/44 kD, Observed band size: 59 kD.