You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1294344 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to TGF beta 1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
Dilution range | Western Blot 1:500 Immunofluorescence 1:100–1:200 Immunohistochemistry (Paraffin) 1:50–1:100 |
Conjugation | Unconjugated |
Target | TGF beta 1 |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti TGF beta 1 antibody at 1/500 dilution. Lysates at 60 μg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294344 overnight at 4°C.
Anti TGF beta 1 antibody at 1/500 dilution. Lysates at 60 μg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294344 overnight at 4°C.
Anti TGF beta 1 antibody at 1/500 dilution. Lysates at 60 μg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294344 overnight at 4°C.
Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294344 anti-TGF beta 1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling TGF beta 1 with orb1294344 at 1/100.
Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling TGF beta 1 with orb1294344 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling TGF beta 1 with orb1294344 at 1/100.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling TGF beta 1 with orb1294344 at 1/100.
ELISA, ICC, IF, IHC-P, WB | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Bovine, Canine, Guinea pig, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rabbit | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Equine, Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating