TFF1 / pS2 antibody (MSVA-482M)

• Catalog Number: orb2276216
• Category: Antibodies
• Description: TFF1 / pS2 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-482M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG2a, kappa
• Immunogen: Recombinant fragment (around aa1-88) of human TFF1protein (exact sequence is proprietary)
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P04155
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: BCEI, Breast Cancer Estrogen Inducible Protein, Ga
• Note: For research use only
• Application notes: Positive Control: Stomach: A strong cytoplasmic TFF1 staining should be seen in the gastric surface epithelium. Negative Control: Kidney: TFF1 immunostaining should be absent in all cell types. Cellular Localization: Cytoplasm Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Ductal adenocarcinoma of the pancreas with strong TFF1 staining of all tumor cells.

Duodenal mucosa with a moderate to strong TFF1 positivity of selected goblet cells.

Surface epithelial cells of the stomach showing an intense cytoplasmic TFF1 staining.