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Catalog Number | orb1252701 |
---|---|
Category | Antibodies |
Description | TFF1 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | GE2 |
Tested applications | FC, IF, IHC, WB |
Reactivity | Human, Primate |
Isotype | IgG1, kappa |
Immunogen | Amino acids CFDDTVRGVPWCFYPNTIDVPPEEECEF (57-84) from the C-terminus of human Estrogen inducible protein pS2 was used as the immunogen for this antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues REQUIRES digestion with Pronase E at 1mg/ml in PBS for 15 min at 37°C. Heat Induced Epitope Retrieval is not suitable for this antibody.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | TFF1 |
UniProt ID | P04155 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Trefoil factor 1, Breast cancer estrogen-inducible Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues REQUIRES digestion with Pronase E at 1mg/ml in PBS for 15 min at 37°C. Heat Induced Epitope Retrieval is not suitable for this antibody.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC staining of human breast cancer with Estrogen Inducible Protein pS2 antibody.
IHC testing of FFPE human ovarian carcinoma with Estrogen Inducible Protein pS2 antibody.
SDS-PAGE Analysis of Purified, BSA-Free Estrogen Inducible Protein pS2 Antibody (clone GE2). Confirmation of Integrity and Purity of the Antibody.
FACS, IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
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