TFF1 Antibody (Center)

• Catalog Number: orb1925791
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB55405
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:25, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 9150 Da
• Target: This TFF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the Central region of human TFF1.
• UniProt ID: P04155
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Trefoil factor 1, Breast cancer estrogen-inducible
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-TFF1 Antibody (Center) at 1:2000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: T47D whole cell lysate. Lane 3: SK-BR-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 9 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling TFF1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing secreted staining on MCF-7 cell line. The nuclear counter stain is DAPI (blue).