TFE3 Antibody / Transcription factor E3

• Catalog Number: orb1822639
• Category: Antibodies
• Description: Transcription factor E3 (TFE3) belongs to the basic helix-loop-helix zipper transcription factor family. The TFE3 gene is located on the short arm of the X chromosome, is expressed in various cells of the human body, and participates in the regulation of various genes. The rearrangement of this gene is associated with a variety of tumors, and it is highly expressed in acinar soft tissue sarcoma with TFE3 fusion gene, Xp11.2 translocation/TFE3 gene fusion-related renal cell carcinoma, and in perivascular epithelioid cell tumors. positive expression.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: TFE3/6849R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Recombinant full-length human TFE3 protein was used as the immunogen for the TFE3 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This TFE3 antibody is available for research use only.
• UniProt ID: P19532
• Storage: Aliquot the TFE3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human MiT translocation RCC with TFE3 antibody. Nuclear staining using TFE3/6849R mAb at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC testing of FFPE human clear cell RCC tissue with TFE3 antibody (clone TFE3/6849R) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.