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Catalog Number | orb1822638 |
---|---|
Category | Antibodies |
Description | Transcription factor E3 (TFE3) belongs to the basic helix-loop-helix zipper transcription factor family. The TFE3 gene is located on the short arm of the X chromosome, is expressed in various cells of the human body, and participates in the regulation of various genes. The rearrangement of this gene is associated with a variety of tumors, and it is highly expressed in acinar soft tissue sarcoma with TFE3 fusion gene, Xp11.2 translocation/TFE3 gene fusion-related renal cell carcinoma, and in perivascular epithelioid cell tumors. positive expression. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | TFE3/6849R |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | Recombinant full-length human TFE3 protein was used as the immunogen for the TFE3 antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This TFE3 antibody is available for research use only. |
UniProt ID | P19532 |
Storage | Aliquot the TFE3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human MiT translocation RCC with TFE3 antibody. Nuclear staining using TFE3/6849R mAb at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC testing of FFPE human clear cell RCC tissue with TFE3 antibody (clone TFE3/6849R) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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