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Catalog Number | orb344660 |
---|---|
Category | Antibodies |
Description | TEV Protease antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Other |
Isotype | IgG |
Immunogen | This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant MBP-and-poly-His-tagged auto inactivation-resistant mutant TEV Protease. |
Concentration | 1.1mg/mL |
Dilution range | ELISA: 1:1,500-1:27,000, WB: 1:500 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. Assay by western blot showed no reactivity to MBP. |
Conjugation | Unconjugated |
UniProt ID | P04517 |
NCBI | NP_734212.1 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-TEV Protease Antibody, Tobacco Etch Vi Read more... |
Note | For research use only |
Application notes | This protein A purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kDa in size corresponding to TEV by western blotting in the appropriate cell lysate or extract. Anti-TEV protease may be useful to detect residual TEV protease in preparations of recombinant proteins in which that protease may interfere with downstream manipulations. |
Expiration Date | 12 months from date of receipt. |
Ecoli lysate containing recombinant TEV protease was loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 1% BSA in TBS for 30 min at ambient. The membrane was probed with the primary antibody at a 1:1000 dilution in 1% BSA/TBS overnight at 4°C. For detection HRP conjugated Gt-a-Rabbit IgG (p/n orb347654) was used at a 1:40000 dilution for 30 min at ambient and data generated with FemtoMax™ enhanced chemiluminescent reagent.
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