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Catalog Number | orb2276439 |
---|---|
Category | Antibodies |
Description | TdT antibody validated for immunohistochemistry on 76 different Normal Tissues |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | Biorbyt-453R |
Tested applications | IHC |
Reactivity | Human |
Isotype | IgG |
Dilution range | 1:75-1:150 |
Conjugation | Unconjugated |
UniProt ID | P04053 |
Storage | Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required. |
Alternative names | Deoxynucleotidyltransferase terminal; DNTT; Nucleo Read more... |
Note | For research use only |
Application notes | Positive Control: Thymus: At least a moderate nuclear immunostaining should be seen in virtually all cortical lymphocytes of the normal thymus. Negative Control: Thymus: The vast majority of the medullary lymphocytes of the normal thymus should be negative. ; Tonsil: The vast majority of lymphoid cells and all epithelial cells should be negative. Cellular Localization: Nucleus Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. |
Expiration Date | 12 months from date of receipt. |
Merkel cell carcinoma with moderate to strong TdT immunostaining in > 80% of tumor cells.
Merkel cell carcinoma with strong TdT positivity in < 5% of tumor cells.
Strong TdT immunostaining in a thymoma.
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