TdT antibody

• Catalog Number: orb2276438
• Category: Antibodies
• Description: TdT antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-453R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Dilution range: 1:75-1:150
• Conjugation: Unconjugated
• UniProt ID: P04053
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Deoxynucleotidyltransferase terminal; DNTT; Nucleo
• Note: For research use only
• Application notes: Positive Control: Thymus: At least a moderate nuclear immunostaining should be seen in virtually all cortical lymphocytes of the normal thymus. Negative Control: Thymus: The vast majority of the medullary lymphocytes of the normal thymus should be negative. ; Tonsil: The vast majority of lymphoid cells and all epithelial cells should be negative. Cellular Localization: Nucleus Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Merkel cell carcinoma with moderate to strong TdT immunostaining in > 80% of tumor cells.

Merkel cell carcinoma with strong TdT positivity in < 5% of tumor cells.

Strong TdT immunostaining in a thymoma.