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Catalog Number | orb371661 |
---|---|
Category | Antibodies |
Description | TCP1 delta/CCT4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, IHC-Fr, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human CCT4 recombinant protein (Position: R452-R539). Human CCT4 shares 97.7% amino acid (aa) sequence identity with both mouse and rat CCT4. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 57924 MW |
UniProt ID | P50991 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | T-complex protein 1 subunit delta;TCP-1-delta;CCT- Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of PC-3 cells using anti-CCT4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control
Flow Cytometry analysis of U251 cells using anti-CCT4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of K562 cells using anti-CCT4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of CCT4 using anti-CCT4 antibody.Lane 1:human HeLa cell;2:human U20S cell;3:human SW620 cell;4:human MCF-7 cell;5:rat brain tissue;6:rat liver tissue;7:mouse brain tissue;8:mouse liver tissue.
IF analysis of CCT4 using anti-CCT4 antibody.CCT4 was detected in immunocytochemical section of U20S cell.
IHC analysis of CCT4 using anti-CCT4 antibody.CCT4 was detected in paraffin-embedded section of mouse kidney tissues.
IHC analysis of CCT4 using anti-CCT4 antibody.CCT4 was detected in paraffin-embedded section of rat kidney tissues.
IHC analysis of CCT4 using anti-CCT4 antibody.CCT4 was detected in paraffin-embedded section of human mammary cancer tissues.
IHC analysis of TCP1 delta using anti-TCP1 delta antibody.TCP1 delta was detected in frozen section of rat brain tissue.
IHC analysis of TCP1 delta using anti-TCP1 delta antibody.TCP1 delta was detected in immunocytochemical section of SHG-44 cell.
IHC analysis of TCP1 delta using anti-TCP1 delta antibody.TCP1 delta was detected in immunocytochemical section of Neuro-2a cell.
IHC analysis of TCP1 delta using anti-TCP1 delta antibody.TCP1 delta was detected in frozen section of mouse brain tissue.
IHC analysis of TCP1 delta using anti-TCP1 delta antibody.TCP1 delta was detected in immunocytochemical section of LOVO cell.
IHC analysis of TCP1 delta using anti-TCP1 delta antibody.TCP1 delta was detected in immunocytochemical section of NRK cell.
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