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Catalog Number | orb371726 |
---|---|
Category | Antibodies |
Description | TCP1 beta/CCT2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human CCT2 recombinant protein (Position: E414-C535). Human CCT2 shares 95.9% and 93.4% amino acid (aa) sequence identity with mouse and rat CCT2, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 57488 MW |
UniProt ID | P78371 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | T-complex protein 1 subunit beta;TCP-1-beta;CCT-be Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U251 cells using anti-CCT2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of CCT2 using anti-CCT2 antibody.Lane 1:rat testis tissue;2:mouse spleen tissue;3:SW620 cell.
IF analysis of CCT2 using anti-CCT2 antibody.CCT2 was detected in immunocytochemical section of U2OS cell.
IF analysis of CCT2 using anti-CCT2 antibody.CCT2 was detected in immunocytochemical section of U20S cell.
IHC analysis of CCT2 using anti-CCT2 antibody.CCT2 was detected in paraffin-embedded section of mouse gaster tissues.
IHC analysis of CCT2 using anti-CCT2 antibody.CCT2 was detected in paraffin-embedded section of rat gaster tissues.
IHC analysis of CCT2 using anti-CCT2 antibody.CCT2 was detected in paraffin-embedded section of human testis tissues.
ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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