TCF4 Antibody

• Catalog Number: orb606621
• Category: Antibodies
• Description: Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4). It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TCF4/1705
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A portion of amino acids 365-671 from the human protein was used as the immunogen for this TCF4 antibody.
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This TCF4 antibody is available for research use only.
• UniProt ID: P15884
• Storage: Store the TCF4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the TCF4 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with TCF4 antibody (clone TCF4/1705). Predicted molecular weight ~71 kDa.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using TCF4 antibody (clone TCF4/1705). These results demonstrate the foremost specificity of the TCF4/1705 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free TCF4 antibody (clone TCF4/1705) as confirmation of integrity and purity.

IHC staining of FFPE human bladder with TCF4 antibody (clone TCF4/1705). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.