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TbHsp70 Antibody: HRP
Catalog Number: orb535203
| Catalog Number | orb535203 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody against TbHsp70 conjugated to HRP |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Plant |
| Immunogen | Synthetic peptide from the mid-protein of Trypanosoma brucei brucei TbHsp70 (aa. 200-300) |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000) |
| Conjugation | HRP |
| MW | 71 kDa |
| Target | TbHsp70 |
| UniProt ID | P11145 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O |
| Alternative names | TbHsp70 antibody, Heat shock 70 kDa protein 4 anti Read more... |
| Note | For research use only |
| Application notes | #REF! |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~71 kDa TbHsp70 protein using Rabbit Anti-TbHsp70 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37°C. Lane 3: Cells incubated for 1 hour at 42°C. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TbHsp70 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71 kDa.
